PART Ⅰ : STRUCTURE AND FUNCTION OF BIOMACROMOLECULE Chapter 1 Structure and Function of Protein SECTION 1 THE COMPOSITION OF PROTEIN 1.1 Amino acid 1.2 Peptide 1.3 Some small polypeptides have biological activity SECTION 2 MOLECULAR STRUCTURE OF PROTEINS 2.1 Primary structure 2.2 Secondary structure 2.3 Tertiary structure 2.4 Major noncovalent bond 2.5 Quaternary structure SECTION 3 RELATION BETWEEN STRUCTURE AND FUNCTION OF PROTEINS 3.1 Primary structure and potential function of protein 3.2 Spatial structure shows the function of protein SECTION 4 CHEMICAL AND PHYSICAL PROPERTIES OF PROTEINS 4.1 Ultraviolet absorption spectrum of protein 4.2 Isoelectric point (pI) 4.3 Denaturation of protein SECTION 5 ISOLATION, PURIFICATION AND SEQUENCING OF PROTEINS 5.1 Dialysis and ultrafiltration 5.2 Precipitation with acetone, salting precipitation and immunoprecipitation 5.3 Chromatographic separations 5.4 Electrophoresis 5.5 Ultracentrifugation SUMMARY Chapter 2 Chemistry of Nucleic Acids SECTION 1 MOLECULAR COMPOSITION OF NUCLEIC ACIDS 1.1 Basic components 1.2 Nucleosides 1.3 Mononucleotides SECTION 2 MOLECULAR STRUCTURES OF DNA 2.1 Primary structure of nucleic acid 2.2 Chargaff's rules 2.3 Secondary structure of DNA SECTION 3 PHYSICO-CHEMICAL PROPERTIES OF NUCLEIC ACIDS 3.1 Viscosity 3.2 Ultraviolet absorption 3.3 Denaturation and renaturation of DNA 3.4 Hydrolysis of acid or alkali SECTION 4 STRUCTURES OF RNA 4.1 Messenger RNA 4.2 Transfer RNA 4.3 Ribosomal RNA SUMMARY Chapter 3 Enzymes SECTION 1 THE MOLECULAR COMPOSITION AND FUNCTION OF ENZYMES 1.1 The molecular composition of enzymes 1.2 The active center of enzymes 1.3 Many enzymes require cofactors SECTION 2 PROPERTIES AND MECHANISM OF ENZYME CATALYSIS 2.1 Remarkable properties of enzymes 2.2 Mechanism of enzyme catalysis SECTION 3 THE KINETICS OF ENZYME CATALYSIS 3.1 Catalysis occurs at the active site 3.2 Multiple factors affect the rates of enzyme-catalyzed reactions 3.3 Initial rate is proportionate to enzyme concentration 3.4 Substrate concentration affects reaction rate 3.5 The Michaelis-Menten equations model the effects of substrate concentration 3.6 Kinetic analysis distinguishes competitive from noncompetitive inhibition SECTION 4 ZYMOGENS AND ISOZYMES 4.1 Zymogens and zymogen activation 4.2 Isozymes are physically distinct forms of the same catalytic activity …… PART Ⅱ : METABOLISM PART Ⅲ : GENE EXPRESSING PART Ⅳ : SPECIAL TOPICS
鄂公網(wǎng)安備 42010302001612號(hào) 