蛋白質組學研究的第一步是要把蛋白質從復雜的大分子混合物中分離純化出來。就此，本書提供了全面的背景資料、理論、實驗方案和實驗結果的優(yōu)化方法，以及疑難解答，包括專門用于蛋白質組學研究的制備、色譜層析和電泳技術，用于結構蛋白質組學研究的蛋白質純化分析技術等。本書是冷泉港實驗室出版社最新推出的Purifying Proteins for Proteomics：A Laboratory Manual的英文影印版，是《蛋白質與蛋白質組學實驗指南》的姐妹篇，秉承了冷泉港實驗手冊一貫的先進性、實用性和權威性，內(nèi)容豐富，信息全面，方案設計完善，技術適用范圍較廣。＜br＞本書是遺傳學、分子生物學研究者從基因組學、基因型研究轉向蛋白質組和表型研究的必備工具書，適于生物化學、分子生物學、細胞生物學、遺傳學、免疫學、蛋白質組學、功能基因組學等生命科學相關領域研究院所、高校相關院系、實驗室的教師、研究生、科研人員，以及生物技術企業(yè)的研發(fā)者和決策者參考使用。
作者簡介
暫缺《蛋白質純化實驗指南（用于蛋白質組學研究影印版）》作者簡介
圖書目錄
Preface Purifying Proteins for Proteomics Companion Web Site Section Ⅰ:Preparing Proteins for Proteomic Analysis 1 Role of Separation Science in Proteomics 2 Strategies for Purifying Proteins Section Ⅱ:Chromatographic Methods for Preparing Proteins for Proteomics 3 Introduction to Chromatographic Methods for Protein and Peptide Purification 4 Multidimensional Chromatography of intact Proteins 5 High-throughput Screening of Soluble Recombinant Proteins 6 lon-exchange Chromatography 7 Size-exclusion Chromatography 8 Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification 9 Hydrophobic Interaction Chromatography 1 Affinity and Immunoaffinity Chromatography 11 Dye-Ligand Affinity Chromatography in Proteomic Research 12 Immobilized Metal-ion Affinity Chromatography 13 Protein Chromatography on Hydroxyapatite 14 Chromatofocusing Section Ⅲ:Electrophoretic Methods for Preparing Proteins for Proteomics 15 Introduction to Strategies Based on Electrophoresis for Separating Proteins 16 Separation of Proteins Using Two-dimensional Gel Electrophoresis 17 Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in a MCE 18 An Electrophoresis Method for Purifying Proteins:The Gradiflow BF4 Instrument 19 Protein Purification Using Free-flow Electrophoresis Section Ⅳ:Analysis of Purified Proteins Prior to Structural Proteomics 2 Introduction to Methods for Characterizing Purified Proteins 21 Characterization of Proteins by Mass Spectrometry 22 Analytical Ultracentrifugation:Protein Size,Conformation,and Interactions 23 Measuring the Conformational Stability of a Protein 24 Combining Surface Plasmon Resonance with Mass Spectrometry:ldentifying Binding Partners and Characterizing Interactions 25 Analysis of Carbohydrate from Glycoproteins Appendices 1.An Overview of Protein Structure 2.Measuring the Concentration of Proteins 3.Concentrating Solutions of Proteins 4.Stabilization of Proteins for Storage 5.One-dimensional Polyacrylamide Gel Electrophoresis 6.Troubleshooting Guides 7.Cautions Index
鄂公網(wǎng)安備 42010302001612號 